Biological Materials Spill Procedures

• Planning for spills
• Spill inside a biosafety cabinet
• Small spill outside a biosafety cabinet
• Large (>500 ml) spill outside a biosafety cabinet

Planning for Spills

The consequences of any spill of biological material can be minimized by performing all work on plastic-backed absorbent liner to absorb spills. A simple spill kit should be readily available and should include the following items:

• chlorine bleach or some other concentrated disinfectant
  
• package or roll of paper towels
  
• autoclavable bag
  
• latex or nitrile gloves
  
• forceps for picking up broken glass

Report spills to your supervisor. Contact the Biosafety Officer for further information.

Spills Inside a Biological Safety Cabinet

1. LEAVE THE CABINET TURNED ON.
   
2. Put on gloves and a lab coat.
   
3. Spray or wipe cabinet walls, work surfaces, and equipment with disinfectant equivalent to a 1:10 bleach solution. If necessary, flood the work surface, as well as drain pans and catch basins below the work surface, with disinfectant.
   
4. Wait at least 20 minutes.
   
5. Soak up disinfectant and spill with paper towels. Drain catch basin into a container. Lift front exhaust grill and tray and wipe all surfaces. Ensure that no paper towels or solid debris are blown into the area beneath the grill.
   
6. Autoclave al clean-up materials before disposal in the biohazardous waste container.
   
7. Wash hands and any exposed surfaces thoroughly after the clean-up procedure.

Small Spill Outside a Biological Safety Cabinet

(spill that can be covered by a few paper towels)

1. Put on gloves and a lab coat.
   
2. Cover spill with paper towels and gently apply disinfectant, proceeding from the outer edge of the spill to its center.
   
3. Leave in place for at least 20 minutes
   
4. Pick up the towels and discard into a biohazard container. Use forceps to pick up any broken glass and place them into a sharps container.
   
5. Re-wipe the spill area with disinfectant.
   
6. Remove gloves and thoroughly wash hands.
   

Large Spill of BL2 Material Outside a Biological Safety Cabinet

(more than 500 ml)

1. HOLD YOUR BREATH AND LEAVE THE ROOM IMMEDIATELY.
   
2. Warn others to stay out of the spill area to prevent spread of contamination.
   
3. Post a sign on the door warning others of the biological materials spill.
   
4. Remove any contaminated clothing and put it into a biohazard bag for later autoclaving.
   
5. Wash hands and exposed skin and inform your PI or supervisor about the spill.
   
6. Put on protective clothing (lab coat, gloves, mask, eye protection, shoe covers) and assemble clean-up materials.
   
7. Wait 30 minutes before re-entering the contaminated area to allow dissipation of aerosols.
   
8. Cover the spill with paper towels and gently apply disinfectant, proceeding from the outer edge of the spill to its center.
   
9. Leave in place for at least 20 minutes.
   
10. Collect all treated materials and discard in a biohazard container. Use forceps to pick up any broken glass and place in a sharps container.
    
11. Re-wipe the spill area with disinfectant.
    
12. Remove glove and wash hands thoroughly.