Diaminobenzidine (DAB) Disposal

While diaminobenzidine (DAB) has not been the subject of in-depth carcinogenicity studies, it is a known mutagen. A mutagen is a physical or chemical agent that can induce changes in the genetic information of an organism. Further, all members of the benzidine family that have been tested are classified as known or suspect carcinogens and the US National Toxicology Program (NTP) considers all benzidine derivatives carcinogens.

TREATMENT OF DAB SOLUTIONS IS NOT EFFECTIVE...

It is a common misconception that simply “treating” DAB solution with sodium hypochlorite (household bleach) serves to detoxify the solution, rendering it non-mutagenic. While hypochlorite may alter the DAB molecule, the resulting byproducts also are mutagenic.

Packaging Solutions for Disposal

Collect DAB wastes in a container appropriate to handle the typical quantities generated in your lab. For example, if your lab only generates a half liter of solution per month, don’t store the waste in a five gallon carboy. Be sure to label your DAB solution with a fully completed Hazardous Waste label and bring the container to your building’s chemical waste collection site when full.

Disposal of DAB Contaminated Debris

Collect DAB contaminated wastes (pipette tips, bench paper, towels, empty containers formerly containing DAB solutions, etc.) and place in a medical waste box labeled "Incinerate Only." Medical waste boxes and liners are available through your department's Building Services representative. When full, seal the liner and box top with packaging tape and place outside your lab door. If the laboratories in your building do not routinely generate medical waste, you may be required to call the Facilities Customer Service Line (8-8000) and arrange for pick-up.

Contact EHS at 258-5294 for more information.